Fecha/hora: 20/05/2024 14:08:42

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	18/09/2014	
Fecha de la última actualización: 	26/11/2019	
Autoria: 	SOTELO, M.; IRISARRI, P.; LORITE, M.J.; CASARETTO, E.; REBUFFO, M.; SANJUAN, J.; MONZA, J.	
Afiliación: 	MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Diversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay.	
Año de publicación: 	2012	
Fuente/Imprenta: 	Applied Soil Ecology,Volume 49, September 2011, Pages 197-207.	
ISSN: 	0929-1393	
DOI: 	10.1016/j.apsoil.2011.05.005	
Idioma: 	Inglés	
Notas: 	Article history: Article history: Received 6 October 2010/Received in revised form 12 May 2011/Accepted 13 May 2011.	
Contenido: 	Abstract:
Diversity of rhizobia nodulating Lotus corniculatus grown in geographical regions with different rainfall regimes in northern and southern Uruguay, was estimated using 168 root nodule isolates. ERIC-PCR analysis revealed no correlation between observed fingerprints and the geographical origin of isolates. Despite the commercial strain U510 has been used for decades to inoculate L. corniculatus, none of the isolates corresponded to this strain. Phylogenetic analyses using 16S rRNA and atpD genes, and ITS sequences clustered all the isolates within genus Mesorhizobium. A great majority of the isolates likely belong to the species M. huakuii, as does the commercial strain U510. The remaining isolates were closely related to either M. septentrionale or M. caraganae. Although no M. loti-like bacteria were identified, all isolates carried symbiotic genes closely related to M. loti and other narrow host range Lotus rhizobia. A significant portion of the Uruguayan isolates were as efficient as the reference strain U510 in symbiosis with L. corniculatus. A few of the isolates were also capable of nitrogen fixation in symbiosis with L. uliginosus, albeit with lower efficiency than reference strains. Our results indicate that rhizobia nodulating L. corniculatus in Uruguay are genetically and phenotypically diverse, and that the commercial strain U510 is probably not adapted to survive the Uruguayan edaphoclimatic conditions.	
Palabras-Clave: 	16S RRNA; ATPD; ITS; MESORHIZOBIUM; NIFH; NODC.	
Thesagro: 	LEGUMINOSAS FORRAJERAS; LOTUS CORNICULATUS; LOTUS ULIGINOSUS; RIZOBIOS.	
Categoría del tema: 	F01 Cultivo	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (100163-1) - PXI - AP - DD - PP/Applied Soil Ecology/2012 -  -  - DIS